My name is Sebastian. I’m a structural biologist based in Kansas City, Kansas, USA. Here are my notes, protocols, and sometimes my thoughts.

This was originally designed as a reference for myself and as such is organized in a way that makes sense to me. References are included when available.

Protocols includes pages on protein expression and purification, recipes, buffers, and tissue culture.

Notes includes pages on basic Linux commands, cryo-EM data processing (RELION and CryoSPARC), and molecular visualization tools (ChimeraX, Chimera, PyMol).

This is very much a work in progress, and I make updates when I have time. If you think I should add something (or correct something) do contact me.

Directory

Protocols

Notes

Other